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KMID : 0861020110260040139
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Korea Journal of Herbology
2011 Volume.26 No. 4 p.139 ~ p.147
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Effects of Aqueous Extract of Diospyros Kaki Calyx on Anti-thrombotic Activity in vitro and in vivo
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Baek Kyung-Min
Roh Seong-Soo
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Abstract
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Objectives: The aim of this study is to research an anti-thrombus effect by Diospyros Kaki Calyx.
Methods: The healthy human plasma were gained and used in vitro study such as factor X activity (FXa)
inhibition, prothrombinase inhibition, prothrombin time (PT) and activated partial thromboplastin time. Fifteen
SD rats were divided into three groups；intact control group (orally administrated with distilled water 5ml/kg)
and two experimental group treated with extract of diospyros kaki calyx (EKC). Experimental rats were orally
600 mg/kg concentration of EKC and 200 mg/kg concentration of EKC. After an hour from administration, we
anesthetized rats and made arteriovenous (AV) shunt rat models to study weight of thrombus, took whole blood
to study content of thromboxane B2 and blood clotting time.
Results: In vitro, EKC significantly increased inhibitory activity of FXa, prothrombinase compared with intact
control group (*P<0.05). PT and aPTT were increased in EKC treated (600 mg/kg) group compared with intact
control group (*P<0.05). In vivo, blood clotting time of experiment group treated with EKC 600 mg/kg were
significantly increased compare with that of intact control group (p<0.05) and content of thromboxane B2 was
significantly decreased in group treated with EKC 600 mg/kg in serum. The weight of thrombus were
significantly reduced in group treated with EKC 600 mg/kg compared with intact control group (p<0.05). But in
vivo experiment study, those parameters of group treated with EKC 200 mg/kg were relatively decreased
compared with those of intact control group without statistical significance.
Conclusions: EKC has an antithrombic activity because of inhibition internal course such as FXa and
prothrombin. And EKC inhibited a hole blood clotting in vivo experiment by low content of thromboxane B2.
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KEYWORD
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Diospyros Kaki Calyx, antithromboic activity, factor Xa assay, prothrombin time assay, thromboxane B2, arteriovenous shunt modle
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